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KMID : 0368420220650010065
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Journal of Plant Biology
2022 Volume.65 No. 1 p.65 ~ p.74
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Ca2+/Calmodulin Activates an MAP Kinase Through the Inhibition of a Protein Phosphatase (DsPTP1) in Arabidopsis
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Kim Kyung-Eun
Nguyen Nhan Thi
Kim Sun-Ho
Bahk Sung-Hwa
Cheong Mi-Sun
Park Hyeong-Cheol
Lee Kyun-Oh
Hong Jong-Chan
Chung Woo-Sik
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Abstract
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Mitogen-activated protein kinases (MPKs) play roles as critical signal components in the environmental stress responses and developmental processes in plants. Calcium ion (Ca2+) is one of the most essential ubiquitous intracellular second messengers involved in many signal transduction pathways in plants. It was previously known that MPKs are activated by the increasing Ca2+ concentration. However, the mechanism of how Ca2+ activates MPKs is not elucidated yet. In this study, we revealed that Ca2+ could activate MPK signaling pathway via inhibiting the activity of a dual-specificity protein phosphatase1 (DsPTP1) by Ca2+/calmodulin (CaM). We showed that DsPTP1 directly interacts with MPK6 in vitro and in vivo. DsPTP1 was able to inactivate the active MPK6 by dephosphorylation. Interestingly, the DsPTP1-mediated dephosphorylation of MPK6 was strongly inhibited by Ca2+/CaM. Moreover, this inhibition was caused by the binding of CaM to the calmodulin-binding domain II (CaMBDII) of DsPTP1. This study implies that Ca2+/CaM is involved in the activation of MPKs through the inhibition of DsPTP1.
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KEYWORD
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Arabidopsis, Calcium, Calmodulin, Kinase, Phosphatase, Stress
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